Evaluation of commercially available reagents as a reference material for intramolecular carbon isotopic measurements of acetic acid

Autor:	Makiko Kikuchi, Ryota Hattori, Alexis Gilbert, Satoshi Hirano, Keita Yamada, Nariaki Wasano, Naohiro Yoshida
Rok vydání:	2014
Předmět:	Chemistry Organic Chemistry Inorganic chemistry chemistry.chemical_element Carbon-13 NMR Mass spectrometry Analytical Chemistry Isotopic signature Acetic acid chemistry.chemical_compound Isotope fractionation Intramolecular force Carbon Sodium acetate Spectroscopy
Zdroj:	Rapid Communications in Mass Spectrometry. 28:1821-1828
ISSN:	0951-4198
Popis:	RATIONALE Recent advances in analytical techniques for the intramolecular carbon isotopic ratio measurement of some organic compounds have provided important information on carbon cycles in biochemistry, organic geochemistry and food chemistry. These advances have made it necessary to prepare intramolecular isotopic reference materials (RMs) to use for inter-laboratory calibration and/or inter-calibration among different analytical methods. METHODS We evaluated the feasibility of preparing RMs using commercially available reagents for intramolecular carbon isotopic ratio measurement of acetic acid. The intramolecular carbon isotopic distribution of nine acetic acid and four sodium acetate reagents was determined with high precision using off-line pyrolysis combined with gas chromatography–combustion–isotope ratio mass spectrometry (GC-C-IRMS). We also evaluated the potential alteration in the isotopic signature of acetic acid reagents by evaporation. RESULTS The intramolecular carbon isotopic distributions for the acetic acid and sodium acetate reagents were determined with a precision of better than 0.45‰. We found that the isotopic values of these reagents spanned the carbon isotopic range of acetic acid in biological and environmental samples. We also found that the isotope fractionation associated with the evaporation of acetic acid occurs solely on the methyl position, the carboxyl position being unaffected. CONCLUSIONS These commercially available reagents will be used as RMs in the future for inter-laboratory calibration and/or inter-calibration with another intramolecular isotopic measurement technique, namely quantitative 13C NMR. In cases where acetic acid is being used as a RM, its storage must be carefully controlled to prevent evaporation. Copyright © 2014 John Wiley & Sons, Ltd.
Databáze:	OpenAIRE
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