A system for ω-transaminase mediated (R)-amination usingl-alanine as an amine donor

Autor:	Desiree Pressnitz, Nina Richter, Wolfgang Kroutil, Judith E. Farnberger, Horst Lechner, Ferdinand Zepeck
Rok vydání:	2015
Předmět:	Alanine animal structures Chemistry Atom economy Alanine racemase Environmental Chemistry Organic chemistry Amine gas treating Enantiomer Pollution Amination Catalysis Transaminase
Zdroj:	Green Chemistry. 17:2952-2958
ISSN:	1463-9270 1463-9262
DOI:	10.1039/c4gc02363c
Popis:	Chiral amines are important building blocks for fine chemicals and pharmaceuticals. Consequently, various biocatalytic routes in particular using ω-transaminases (ω-TAs) have been developed recently. Although catalysts for the synthesis of both enantiomers are available, the application of alanine dependent (R)-selective ω-TAs is less favourable due to the requirement of the more expensive D-alanine as an amine donor. Here we describe an efficient method for (R)-amination using ω-TAs in combination with an alanine racemase (AlaR). In this case, the readily available L-alanine can be used as an amine donor leading to improved atom efficiency and significantly reduced costs.
Databáze:	OpenAIRE
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