| Autor: |
Hong-Bo Gao, Xin-Li Xia, Qing Hu, Qiu-Qi Li, Huihong Guo, Wen-Hong Deng, Ting-Ting Wang |
| Rok vydání: |
2013 |
| Předmět: |
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| Zdroj: |
Journal of the Science of Food and Agriculture. 94:482-488 |
| ISSN: |
0022-5142 |
| DOI: |
10.1002/jsfa.6273 |
| Popis: |
BACKGROUND Xanthoceras sorbifolia Bunge is a valuable oilseed tree that has linoleic acid-rich seed oil. Microsomal oleate desaturase (FAD2; EC 1.3.1.35) is responsible for the conversion of oleic acid to linoleic acid during fatty acid synthesis. In this study, XsFAD2 was cloned from developing embryos of X. sorbifolia. RESULTS XsFAD2 contained three histidine boxes, a C-terminal endoplasmic reticulum retrieval motif, and five putative transmembrane domains representing the characteristics of membrane-bound fatty acid desaturase. XsFAD2 expression in yeast cells resulted in linoleic acid (18:2) and palmitolinoleic acid (16:2) production, confirming the biological activity of the enzyme encoded by XsFAD2. These fatty acids are not normally present in wild-type yeast. Phylogenetic analysis indicated that XsFAD2 is located in a subgroup of FAD2 enzymes specifically or highly expressed in developing seeds. The expression level of XsFAD2 in seeds was much higher than those in leaves and petals. Furthermore, XsFAD2 expression pattern correlated well with linoleic acid accumulated in seeds. CONCLUSION Results suggested that XsFAD2 is responsible for the high linoleic acid content in X. sorbifolia seed oil. This study provides insight on the regulation mechanism of fatty acid synthesis in X. sorbifolia seeds and a valuable gene for improving the oil quality in oilseed trees. © 2013 Society of Chemical Industry |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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