‘Live and Large’: Super-Resolution Optical Fluctuation Imaging (SOFI) and Expansion Microscopy (ExM) of Microtubule Remodelling by Rabies Virus P Protein

Autor:	Gregory W. Moseley, Donna R. Whelan, Sam Duwé, Ashley M. Rozario, Fabian U. Zwettler, Aaron Brice, Peter Dedecker, Toby D. M. Bell, Riley B. Hargreaves, Markus Sauer
Rok vydání:	2020
Předmět:	0303 health sciences Chemistry Rabies virus Resolution (electron density) New materials General Chemistry 010402 general chemistry medicine.disease_cause 01 natural sciences Superresolution 0104 chemical sciences Cell biology 03 medical and health sciences Microtubule Microscopy medicine 030304 developmental biology
Zdroj:	Australian Journal of Chemistry. 73:686
ISSN:	0004-9425
DOI:	10.1071/ch19571
Popis:	The field of super-resolution microscopy continues to progress rapidly, both in terms of evolving techniques and methodologies as well as in the development of new multi-disciplinary applications. Two current drivers of innovation are increasing the possible resolution gain and application in live samples. Super-resolution optical fluctuation imaging (SOFI) is well suited to live samples while expansion microscopy (ExM) enables obtainment of sub-diffraction information via conventional imaging. In this Highlight we provide a brief outline of these methods and report results from application of SOFI and ExM in our on-going study into microtubule remodelling by rabies virus P proteins. We show that MT bundles in live cells transfected with rabies virus P3 protein can be visualised using SOFI in a time-lapse fashion for up to half an hour and can be expanded using current Pro-ExM protocols and imaged using conventional microscopy.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::1154e1b170ee34c5fe37b3d15fafb572 https://doi.org/10.1071/ch19571 Zobrazit plný text záznamu Plný text ve formátu PDF Plný text ve formátu HTML
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