Evaluation of Nosema ceranae spore-specific polyclonal antibodies

Autor:	Eduardo Saldivar, Thomas C. Webster, Katherine A. Aronstein
Rok vydání:	2011
Předmět:	biology fungi Honey bee biology.organism_classification Virology Nosema ceranae Staining Spore Microbiology Nosema Antigen Polyclonal antibodies Insect Science biology.protein Antibody
Zdroj:	Journal of Apicultural Research. 50:145-151
ISSN:	2078-6913 0021-8839
Popis:	SummaryA new genomic antibody (Ab) has been developed against a spore-wall protein SWP-32 of the honey bee intracellular pathogen, Nosema ceranae. In dot blots and Western blots this Ab specifically recognized N. ceranae spore antigens and did not cross-react with N. apis spore lysates, unless blots were overdeveloped. The detection sensitivity depends on both the concentration of the anti-SWP-32 Ab and the concentration of Nosema spores in the lysates. To avoid non-specific staining, we suggest using this new Ab at 1:5000 dilution for detection of 1×103 and higher spore numbers per assay. Considering that a single infected bee can produce over 50 × 106 spores, this level of sensitivity will allow detection of a very low level of Nosema infection in bee colonies.
Databáze:	OpenAIRE
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