Modified agar-based media for culturing Phytophthora infestans

Autor:	Somsiri Sangchote, W R Stevenson, J. Sopee
Rok vydání:	2012
Předmět:	food.ingredient digestive oral and skin physiology food and beverages Plant Science Biology biology.organism_classification Sunflower Spore Horticulture food Insect Science Botany Phytophthora infestans Asian country Agar Oospore Oospore formation
Zdroj:	Phytoparasitica. 40:269-278
ISSN:	1876-7184 0334-2123
Popis:	Studies were undertaken to evaluate locally available subtrates for use in a culture medium for Phytophthora infestans (Mont.) de Bary employing a protocol similar to that used for the preparation of rye A agar. Test media preparations were assessed for growth, sporulation, oospore formation, and long-term storage of P. infestans. Media prepared from grains and fresh produce available in Thailand and Asian countries such as black bean (BB), red kidney bean (RKB), black sesame (BSS), sunflower (SFW) and sweet corn supported growth and sporulation of representative isolates compared with rye A, V8 and oat meal media. Oospores were successfully formed on BB and RKB media supplemented with β-sitosterol. The BB, RKB, BSS and SFW media maintained viable fungal cultures with sporulation ability for 8 months, similar to the rye A medium. Three percent and 33% of 135 isolates failed to grow on V8 and SFW media, respectively.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::114f16db49d0dde97b416125d755d6d7 https://doi.org/10.1007/s12600-012-0218-4 Zobrazit plný text záznamu Plný text ve formátu PDF