Cytogenetic damage and induction of pro-oxidant state in human lymphocytes exposed in vitro to gliphosate, vinclozolin, atrazine, and DPX-E9636
| Autor: | Dino Di Berardino, Maria Rosaria Scarfì, Antonietta Santoro, Olga Zeni, Francesca Salvemini, Matilde Valeria Ursini, Maria Brigida Lioi, R. Barbieri |
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| Rok vydání: | 1998 |
| Předmět: |
Mitotic index
Epidemiology Health Toxicology and Mutagenesis Lymphocyte Sister chromatid exchange Biology medicine.disease_cause Molecular biology chemistry.chemical_compound medicine.anatomical_structure chemistry Biochemistry Toxicity medicine Glucose-6-phosphate dehydrogenase Vinclozolin Atrazine Genetics (clinical) Genotoxicity |
| Zdroj: | Environmental and Molecular Mutagenesis. 32:39-46 |
| ISSN: | 1098-2280 0893-6692 |
| DOI: | 10.1002/(sici)1098-2280(1998)32:1<39::aid-em5>3.0.co;2-6 |
| Popis: | We analyzed chromosome aberrations (CAs), sister chromatid exchanges (SCEs), mitotic index (MI), and glucose 6-phosphate dehydrogenase (G6PD) enzyme activity in human peripheral lymphocytes from three healthy donors exposed in vitro to different concentrations of gliphosate, vinclozolin, atrazine, and DPX-E9636. The pesticides gliphosate, vinclozolin, and atrazine have been studied in a broad range of genetic tests with predominantly conflicting or negative results, whereas little is known about the genotoxicity of DPX-E9636. In our experimental conditions, each chemical compound tested produced a dose-related increase in the percent of aberrant cells and an increase of SCE/cell. Furthermore, at the highest concentrations of vinclozolin, atrazine, and DPX-E9636, we observed a significant reduction of the mitotic index. The increase of G6PD activity in exposed lymphocyte cultures strongly indicated an induction of a pro-oxidant state of the cells as an initial response to pesticide exposure. |
| Databáze: | OpenAIRE |
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