Two-in-One Strategy for Effective Enrichment of Phosphopeptides Using Magnetic Mesoporous γ-Fe2O3 Nanocrystal Clusters
| Autor: | Changchun Wang, Yuting Zhang, Haojie Lu, Ying Zhang, Lulu Li, Jia Guo, Wanfu Ma, Meng Yu |
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| Rok vydání: | 2013 |
| Předmět: | |
| Zdroj: | ACS Applied Materials & Interfaces. 5:614-621 |
| ISSN: | 1944-8252 1944-8244 |
| Popis: | Designed with a two-in-one strategy, the magnetic mesoporous γ-Fe2O3 nanocrystal clusters (m-γ-Fe2O3) have been successfully prepared for integrating the functions of effective enrichment and quick separation of phosphopeptides into a single architecture. First, the mesoporous Fe3O4 nanocrystal clusters (mFe3O4) were synthesized by solvothermal reaction and then were subjected to calcination in air to form m-γ-Fe2O3. The obtained m-γ-Fe2O3 have spherical morphology with uniform particle size of about 200 nm and mesoporous structure with the pore diameter of about 9.7 nm; the surface area is as large as 117.8 m2/g, and the pore volume is 0.34 cm3/g. The m-γ-Fe2O3 possessed very high magnetic responsiveness (Ms = 78.8 emu/g, magnetic separation time from solution is less than 5 s) and were used for the selective enrichment of phosphopeptides for the first time. The experimental results demonstrated that the m-γ-Fe2O3 possessed high selectivity for phosphopeptides at a low molar ratio of phosphopeptides/nonp... |
| Databáze: | OpenAIRE |
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