Continuous-wave near-infrared stimulated-emission depletion microscopy using downshifting lanthanide nanoparticles
| Autor: | Yu Wang, Min Gu, Melgious Jin Yan Ang, Qiming Zhang, Xiangping Li, Liangliang Liang, Zhigao Yi, Bengang Xing, Thang Do Cong, Han Feng, Xian Qin, Lei Zhou, Xiaogang Liu, Ziwei Feng |
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| Rok vydání: | 2021 |
| Předmět: |
Materials science
Biomedical Engineering Nanoprobe Bioengineering 02 engineering and technology 010402 general chemistry 01 natural sciences law.invention law Microscopy General Materials Science Stimulated emission Electrical and Electronic Engineering business.industry Near-infrared spectroscopy STED microscopy 021001 nanoscience & nanotechnology Condensed Matter Physics Laser Photobleaching Atomic and Molecular Physics and Optics 0104 chemical sciences Autofluorescence Optoelectronics 0210 nano-technology business |
| Zdroj: | Nature Nanotechnology. 16:975-980 |
| ISSN: | 1748-3395 1748-3387 |
| Popis: | Stimulated-emission depletion (STED) microscopy has profoundly extended our horizons to the subcellular level1–3. However, it remains challenging to perform hours-long, autofluorescence-free super-resolution imaging in near-infrared (NIR) optical windows under facile continuous-wave laser depletion at low power4,5. Here we report downshifting lanthanide nanoparticles that enable background-suppressed STED imaging in all-NIR spectral bands (λexcitation = 808 nm, λdepletion = 1,064 nm and λemission = 850–900 nm), with a lateral resolution of below 20 nm and zero photobleaching. With a quasi-four-level configuration and long-lived (τ > 100 μs) metastable states, these nanoparticles support near-unity (98.8%) luminescence suppression under 19 kW cm−2 saturation intensity. The all-NIR regime enables high-contrast deep-tissue (~50 μm) imaging with approximately 70 nm spatial resolution. These lanthanide nanoprobes promise to expand the application realm of STED microscopy and pave the way towards high-resolution time-lapse investigations of cellular processes at superior spatial and temporal dimensions. The application of stimulated-emission depletion (STED) microscopy for deep-tissue imaging in the near-infrared optical window is challenged by high cellular autofluorescence. Here the authors present a lanthanide nanoprobe whose electronic configuration enables long-term STED imaging with reduced background noise. |
| Databáze: | OpenAIRE |
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