Role of Oxidative Tissue Injury in the Pathophysiology of Experimentally Induced Equine Laminitis: A Comparison of 2 Models

Autor: G.J. Nuovo, T.A. Burns, A. Pettigrew, T. Westerman, J.K. Belknap, M.R. Watts, C. Yin
Rok vydání: 2011
Předmět:
Zdroj: Journal of Veterinary Internal Medicine. 25:540-548
ISSN: 0891-6640
DOI: 10.1111/j.1939-1676.2011.0706.x
Popis: Background: Oxidative stress reportedly plays a role in sepsis-induced organ dysfunction and failure in many species. In septic horses, laminae are targeted; evidence of laminar oxidative stress has been reported experimentally in the black walnut extract (BWE) model. Carbohydrate (CHO)-induced laminitis may be more similar to clinical sepsis-related laminitis than the BWE model in that animals with CHO-induced disease commonly develop laminar failure. The role of oxidative stress in the CHO model remains unknown. Hypothesis/Objectives: Markers of oxidative stress will be increased in laminae from horses with BWE- and CHO-induced laminitis. Animals: Banked laminar tissue from various time points from animals subjected to BWE (n = 15) and CHO (n = 20) protocols. Methods: Laminar 4-hydroxynonenal (4-HNE) and protein carbonyl content were evaluated by slot blot analysis. Laminar 3-nitrotyrosine (3-NT) immunohistochemistry was performed. Results: The number of laminar 3-NT (+) cells was increased at developmental and Obel grade 1 (OG1) time points in the BWE model (versus control [CON]; P= .013) and lower in OG1 tissues than CON in the CHO model (P= .04). No change in 4-HNE content was observed in the CHO model, and no increase in laminar protein carbonyl content was present in either model (P > .05). Conclusions and Clinical Importance: These results do not support a prominent role for oxidative stress at examined time points in CHO-overload laminitis and support transient oxidative stress in the BWE model. Tissue oxidation does not appear to be a central early pathophysiologic event in CHO-associated laminitis.
Databáze: OpenAIRE
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