Protein binding study of isoflavone, perillyl alcohol and S-ibuprofen by high-performance frontal analysis

Autor:	Dexian Wang, Du Young Choi, Long Mei Jin, Kyung Ho Row
Rok vydání:	2005
Předmět:	Chromatography General Chemical Engineering Perillyl alcohol Sodium Daidzein Genistein chemistry.chemical_element General Chemistry Plateau (mathematics) Human serum albumin chemistry.chemical_compound chemistry Ionic strength Genistin medicine medicine.drug
Zdroj:	Korean Journal of Chemical Engineering. 22:465-469
ISSN:	1975-7220 0256-1115
DOI:	10.1007/bf02719427
Popis:	High-performance frontal analysis (HPFA) was used for a protein binding study of isoflavones (daidzein, genistin, and genistein), enantiomers of perillyl alcohol and S-ibuprofen to human serum albumin (HSA). The analyses were performed on a Develosil and Inertsil 100-Diol-5 column (10 cm×4.6mm). Sodium phosphate solution (pH 7.4, ionic strength 0.17) was used as the mobile phase at a flow rate of 1 ml/min. To ensure the drug to be eluted as a trapezoidal peak with a plateau, injection volumes were each fixed up the zonal profile with an evident plateau appears. The unbound drug concentration was determined from a plateau height of the plateau region after that experimental data were fitted by Scatchard equation. The binding constants (K) and total binding affinities (nK) of drugs to HSA were calculated, respectively.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::11078f9edea93d50ba832da2816bc3e0 https://doi.org/10.1007/bf02719427 Zobrazit plný text záznamu Full text from SpringerLink