Selection of somaclonal variants of oats resistant to Helminthosporium victoriae which produces a host specific toxin, victorin

Autor:	Yukio Sasabe, Toshikazu Tani, Shigeyuki Mayama, Yasuharu Oishi, Ana Paula Ayres Bordin
Rok vydání:	1990
Předmět:	Genetics Toxin Callus formation fungi food and beverages Biology medicine.disease_cause Microbiology Somaclonal variation Tissue culture Germination Shoot Genotype medicine Allele
Zdroj:	Plant tissue culture letters. 7:64-68
ISSN:	1884-7706 0289-5773
Popis:	Somaclonal variation generated in tissue culture of oats was screened for insensitivity to victorin which is a host specific toxin produced by Helminthosporium victoriae. Mature embryos of oats (Iowa X 469) with the sensitive allele Vb (Pc-2) were cultured in MS media with 2, 4-D. Callus formation was best in 3.5mg/l of 2, 4-D. Continuous subcultures for every 2 weeks resulted in the induction of shoot, followed by the formation of root when cultured in hormone-free media. About 600 regenerated oat plants obtained from about 4000 calli were screened for resistance to victorin D which was found as a major toxin secreted during germination. Two regenerants showed toxin-tolerance. The progeny of one of the regenerants were all tolerant to the toxin, whereas that of the other segregated, suggesting that the former genotype is recessively homozygous and the latter is heterozygous. The toxin-insensitive regenerants were also highly resistant to the fungal infection.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::1102fc9cd3985c23a3c3a45e2f4a75d2 https://doi.org/10.5511/plantbiotechnology1984.7.64 Zobrazit plný text záznamu