| Autor: |
W. Wilson Pitt, auWayne F. Johnson, Charles D. Scott |
| Rok vydání: |
1974 |
| Předmět: |
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| Zdroj: |
Journal of Chromatography A. 99:35-42 |
| ISSN: |
0021-9673 |
| DOI: |
10.1016/s0021-9673(00)90844-6 |
| Popis: |
The feasibility of using centrifugal, multicolumn elution chromatography in conjuction with eluate monitoring is being studied. A preliminary prototype system has as many as four columns mounted in a spinning rotor that passes through a stationary photometric detection station. Cuvette windows at the terminal end of each chromatographic column allow photometric monitoring of each column eluate as it passes through the detector. The monitor is capable of associating a given output signal with the appropriate chromatographic column using suitable digital logic. The photometric output can be recorded by a conventional strip-chart recorder or via a computer by printed tabulations. In the present mode of operation a common sample is introduced simultaneously to each column with a single eluent stream being used for all columns. Preliminary tests have been directed toward establishing the best rotor design for hydrodynamic stability and overall operability. The system is being considered for the assay of several serum proteins from a single serum sample utilizing affinity chromatography. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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