Primers designed to amplify a mitochondrial nad1 intron in ponderosa pine, Pinus ponderosa, limber pine, P. flexilis, and Scots pine, P. sylvestris

Autor:	B. R. Kreiser, G. E. Rehfeldt, J. B. Mitton
Rok vydání:	2000
Předmět:	Intron Scots pine General Medicine Biology Dna amplification biology.organism_classification Pinus Limber pine Genetic variation Botany Genetics Indel Agronomy and Crop Science Biotechnology Woody plant
Zdroj:	Theoretical and Applied Genetics. 101:1269-1272
ISSN:	1432-2242 0040-5752
DOI:	10.1007/s001220051606
Popis:	The b/c intron of the mitochondrial nad1 gene, was sequenced to characterize the indel region of ponderosa pine, Pinus ponderosa. The sequence in ponderosa pine was aligned with the sequence in Scots pine, Pinus sylvestris, to design seven primers that are useful for sequencing and for revealing size variation in amplified fragments in ponderosa pine, Scots pine, and limber pine, Pinus flexilis. These primers reveal variability in all three species, and the pattern of variability within ponderosa pine is described by a preliminary survey. The indel region of ponderosa pine contains three distinct elements with lengths of 31, 32, and 34 bp.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::10e399a51a3c8103a69920fd33c9d753 https://doi.org/10.1007/s001220051606 Zobrazit plný text záznamu Full text from SpringerLink