Nucleic Acids Extraction from Laser Microdissected FFPE Tissue Sections

Autor:	Renate Burgemeister
Rok vydání:	2011
Předmět:	chemistry.chemical_compound Chromatography Tissue microarray chemistry Extraction (chemistry) Nucleic acid RNA RNA extraction Biology Molecular biology DNA Microdissection Laser capture microdissection
Zdroj:	Methods in Molecular Biology ISBN: 9781617790546
DOI:	10.1007/978-1-61779-055-3_8
Popis:	Tissue heterogeneity is a common source of unsuccessful experiments. Laser capture microdissection is a tool to prepare homogeneous tissue and cell areas as starting material for reliable and reproducible results as it allows the defined investigation of spatially different tissue areas.Nearly all samples allow the extraction of DNA. Fresh or fresh frozen samples are an ideal source for getting access to high-quality RNA. But also the large archives of formalin-fixed, paraffin-embedded (FFPE) tissue specimens are a valuable source of sample material for RNA extraction. Optimized protocols may help to make the RNA from FFPE material suitable for expression studies.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::10e2613d3ebc9d07ffc23294aca1180c https://doi.org/10.1007/978-1-61779-055-3_8 Zobrazit plný text záznamu