Characterization of an ultrasensitive biosensor based on a nano-Au/DNA/nano-Au/poly(SFR) composite and its application in the simultaneous determination of dopamine, uric acid, guanine, and adenine
| Autor: | Ling Mei Niu, Kao Qi Lian, Si Yuan Bi, Wei Jun Kang, Hong Mei Shi, Yi Bing Wu |
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| Rok vydání: | 2013 |
| Předmět: |
Detection limit
Guanine Metals and Alloys Analytical chemistry Nanoparticle Condensed Matter Physics Surfaces Coatings and Films Electronic Optical and Magnetic Materials Dielectric spectroscopy chemistry.chemical_compound chemistry Colloidal gold Electrode Materials Chemistry Differential pulse voltammetry Electrical and Electronic Engineering Instrumentation Biosensor Nuclear chemistry |
| Zdroj: | Sensors and Actuators B: Chemical. 178:10-18 |
| ISSN: | 0925-4005 |
| DOI: | 10.1016/j.snb.2012.12.015 |
| Popis: | An ultrasensitive method for the simultaneous determination of dopamine (DA), uric acid (UA), guanine (G), and adenine (A) was developed using differential pulse voltammetry with a three-dimensionally distributed Au nanoparticle (GNP)-modified glassy carbon electrode (GCE). The nano-Au/DNA/nano-Au/poly(SFR)/GCE microstructure was characterized by scanning electron microscopy, electrochemical impedance spectroscopy, and atomic force microscopy, showing an anchored three-dimensional distribution of GNPs on the modified electrode. The electrode exhibited ultrasensitive responses to DA, UA, G, and A due to poly(SFR) electrocatalytic activities and the large surface area of the GNPs. All four analytes showed well-defined catalytic oxidation peaks at the modified electrode. DA, UA, G, and A yielded linear ranges from 8.0 × 10−9 M to 1.1 × 10−6 M, 9.0 × 10−8 M to 1.2 × 10−5 M, 9.0 × 10−9 M to 5.0 × 10−6 M, and 6.0 × 10−8 M to 8.0 × 10−7 M, respectively, and detection limits for the analytes were 2.0 × 10−10 M, 8.0 × 10−9 M, 5.0 × 10−10 M, and 4.0 × 10−9 M, respectively. The feasibility of the proposed assay for use in human serum and urine was investigated and satisfying results were obtained. |
| Databáze: | OpenAIRE |
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