Rapid method for determining total glucosinolates in rapeseed by measurement of enzymatically released glucose

Autor:	Clifford A. Smith, Clive Dacombe
Rok vydání:	1987
Předmět:	Nutrition and Dietetics Chromatography biology Myrosinase food and beverages chemistry.chemical_compound Hydrolysis chemistry Biochemistry Activated charcoal Sephadex Glucosinolate biology.protein Glucose oxidase Methanol Agronomy and Crop Science Food Science Biotechnology Peroxidase
Zdroj:	Journal of the Science of Food and Agriculture. 38:141-150
ISSN:	1097-0010 0022-5142
DOI:	10.1002/jsfa.2740380206
Popis:	Glucosinolates were hydrolysed with endogenous myrosinase to liberate glucose by ball-milling whole seed with water. Similar ball-milling with acidified 40% methanol deactivated myrosinase and allowed free glucose in the seed to be measured separately. Extracts were filtered through activated charcoal paper to remove interfering phenolic compounds, and glucose was measured by a glucose oxidase/peroxidase colorimetric procedure. Correction was made for free glucose before calculating total glucosinolate content of the seed. The procedure was simple, reproducible and rapid (30 min) and the addition of exogenous myrosinase was unnecessary. It gave figures in excellent agreement with the more lengthy Sephadex micro-column method and the proposed EEC reference procedure based on temperature-programmed gas-liquid chromatography of trimethylsilyl derivatives formed from enzymatically produced desulphoglucosinolates.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::10be86a93c200fd16342bd786eb850f4 https://doi.org/10.1002/jsfa.2740380206 Zobrazit plný text záznamu Plný text