| Autor: |
Kenneth C. Kuo, T. Phillip Waalkes, Robert W. Zumwalt, Charles W. Gehrke |
| Rok vydání: |
1990 |
| Předmět: |
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| DOI: |
10.1016/s0301-4770(08)61539-0 |
| Popis: |
Publisher Summary This chapter discusses the topic of nucleic acid components as potential biological markers; specifically, modified nucleosides. There have been indications for more than 30 years that cancer patients excrete elevated levels of methylated purines and pyrimidines as well as other modified bases and nucleosides. The origin of these compounds was obscure until the discovery of the modification of transfer RNA. Investigators have also attempted to elucidate cancer markers that could be utilized to predict which patients are more likely to respond to treatment and which patients have a worse prognosis. High performance liquid chromatography with diode array detection (HPLC-UV) has emerged as one of the most popular and powerful techniques for studying the constituents of nucleic acids, especially in complex samples such as physiological fluids and cell extracts. Research on tRNA catabolites in urine and serum/plasma has concentrated on HPLC analysis of the modified nucleosides following isolation of the nucleosides by boronate gel affinity chromatography as the nucleosides are generally the major tRNA catabolic excretion products and are easily isolated by the boronate gel. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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