| Autor: |
Xia Yanchun, Pan Xiaoyi, Zheng Cao, Shen Jinyu, Yin Wenlin, Guoqiang Mi, Cheng-Sai Zang, Ling-Yun Ling, Chun-Feng Wang, Yong-Yi Jia, Yao Jiayun, Zhi-Min Gu, Jian-Lin Guo |
| Rok vydání: |
2019 |
| Předmět: |
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| Zdroj: |
Aquaculture. 506:465-469 |
| ISSN: |
0044-8486 |
| DOI: |
10.1016/j.aquaculture.2019.02.066 |
| Popis: |
The main purpose of the present study was undertaken to isolate bioactive substances from microbial secondary metabolites of Streptomyces gilvosporeu AXY-25 and to determine their antiparasitic effect against Ichthyophthirius multifiliis. A pure compound showing strong anti- I. multifiliis activity was isolated from a culture of S. gilvosporeu AXY-25 by using the method of bioassay-guided isolation. The chemical structure of the active compound was confirmed as natamycin (NAT) by spectral analysis (EI-MS, 1H NMR and 13C NMR). An in vitro anti-parasitic assay demonstrated that 100% of theronts were killed with a concentration of 25.0 mg L−1 NAT for 4 h with an effective concentration (EC50) (95% CI) at 10.9 (10.7–11.1) mg L−1. Similiary, all tomonts were killed by a dose of 25.0 mg L−1. An in vivo anti-parasitic assay showed that the number of I. multifiliis trophonts on the surface of hybrid Erythroculter ilishaeformis in the NAT-treated group were markedly lower when compared to the control group (p |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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