Non-viral siRNA delivery vectors: dendritic molecular transporter and molecular transporter nanovectors for target gene silencing

Autor:	Jenna Donavan, Artez L. Sims, Sharon K. Hamilton, Eva Harth
Rok vydání:	2011
Předmět:	Polymers and Plastics Organic Chemistry Bioengineering Transporter Biological activity Biology Biochemistry Molecular biology Cell biology Cytosol Gene silencing Target protein Linker Intracellular Conjugate
Zdroj:	Polym. Chem.. 2:441-446
ISSN:	1759-9962 1759-9954
DOI:	10.1039/c0py00342e
Popis:	Viable non-viral delivery vectors have been utilized for the intracellular delivery of siRNA duplexes to result in complete target gene silencing. Biologically active siRNA duplexes were attached to a dendritic molecular transporter (MT) or a molecular transporter-polymeric nanoparticle conjugate (MT-NP) via a thiol exchange reaction utilizing a dithiol pyridyl linker. The MT and the MT-NP delivery systems resulted in significant reduction of the target protein (lamin A/C) investigated for a range of conjugate concentrations. Both non-viral systems have proven effective for the delivery of siRNA into the cytosol of live cells and specifically the development of the MT-NP delivery system provides opportunities for targeted delivery of siRNA into selected cell types.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::1098c2f18c0552f9e7f0594355fdfc30 https://doi.org/10.1039/c0py00342e Zobrazit plný text záznamu