Accumulation of ATP by plasma membranes of human and rat hepatocytes induced by some growth factors and phosphatidylcholine
| Autor: | Karelin Aa, Demidova Vs, O. Yu. Abakumova, Globa Ag, V. A. Vishnevskii |
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| Rok vydání: | 1996 |
| Předmět: |
Cell growth
General Medicine Oxidative phosphorylation Mitochondrion Biology General Biochemistry Genetics and Molecular Biology Transmembrane protein Cell biology chemistry.chemical_compound medicine.anatomical_structure Biochemistry chemistry Phosphatidylcholine Hepatocyte medicine V-ATPase Signal transduction |
| Zdroj: | Bulletin of Experimental Biology and Medicine. 121:249-252 |
| ISSN: | 1573-8221 0007-4888 |
| Popis: | Using the method of ATP luminometry it is shown that crude membrane preparations from human and rat hepatocytes accumulate ATP 20–100 nmol/mg protein during a 1-min incubation under conditions of oxidative phosphorylation. Application of appropriate inhibitors shows that a possible contamination of the membrane preparations with mitochondria does not contribute to this ATP accumulation. Phosphatidylcholine, tumor necrosis factor, and cell proliferation factor markedly stimulate the accumulation of ATP by plasma membraneenriched particles isolated from rat and human liver. The hepatocyte plasma membrane is shown to be able to synthesize ATP from inorganic phosphate and ADP using the aerobic mechanism. ATP in the plasma membrane is assumed to participate in the transmembrane signal transduction from growth factors to the cell effector systems. |
| Databáze: | OpenAIRE |
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