In vitro evaluation of the antibacterial activity of amitriptyline and its synergistic effect with ciprofloxacin, sulfamethoxazole–trimethoprim, and colistin as an alternative in drug repositioning

Autor: Vitória Segabinazzi Foletto, Angelita Bottega, Tacieli Fagundes da Rosa, Marissa Bolson Serafin, Rosmari Hörner, Sara de Lima Marion, Catrine de Souza Machado, Vinícius Victor Lorenzoni, Silvana Silveira Coelho, Roberta Filipini Rampelotto
Rok vydání: 2019
Předmět:
Zdroj: Medicinal Chemistry Research. 29:166-177
ISSN: 1554-8120
1054-2523
Popis: In the present study we evaluated the biological activity of amitriptyline, a tricyclic antidepressant, and its antibacterial activity was determined in vitro, individually and in combination with ciprofloxacin, sulfamethoxazole-trimethoprim, and colistin. Being tested against multi drug resistant clinical isolates and American Type Culture Collection (ATCC) standard strains, through the fractional inhibitory concentration index (FICI) and tolerance index. Their ability to cleave plasmid DNA was also analyzed. Amitriptyline showed activity against Gram-positive and Gram-negative bacteria. In combination with colistin, two strains of Klebsiella pneumoniae producing carbepenemase with a minimum inhibitory concentration of 4 μg/mL were prominent. FICI demonstrated synergistic effect in 15 combinations with antibiotics. Amitriptyline was able to cleave the DNA at concentrations of 3.75 and 1.875 mM at pH 7.4 at 50 °C. The results obtained in this study allow us to suggest amitriptyline as a potential antibiotic in drug repositioning.
Databáze: OpenAIRE
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