Isolation and functional characterization of lipase from the thermophilic alkali-tolerant bacterium Thermosyntropha lipolytica

Autor:	Nikolai V. Ravin, Andrey V. Mardanov, Vadim M. Gumerov, Peter M. Kolosov
Rok vydání:	2012
Předmět:	Thermophile Biology medicine.disease_cause biology.organism_classification Applied Microbiology and Biotechnology Biochemistry Hydrolysis Biocatalysis Biodiesel production medicine biology.protein Lipase Thermosyntropha lipolytica Escherichia coli Bacteria
Zdroj:	Applied Biochemistry and Microbiology. 48:338-343
ISSN:	1608-3024 0003-6838
Popis:	As a result of sequencing the genome of the termophilic alkali-tolerant lipolytic bacterium Thermosyntropha lipolytica, the gene encoding a lipase secreted into the medium was identified. The recombinant enzyme was expressed in Escherichia coli. It was isolated, purified, and functionally characterized. The lipase exhibited hydrolytic activity toward para-nitrophenyl esters of various chain lengths, as well as triglycerides, including vegetable oils. The optimal reaction conditions were achieved at temperatures from 70 to 80°C and pH 8.0. This new thermostable lipase may be a promising biocatalyst for organic synthesis; it may find application in the food and detergent industry and biodiesel production.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::108e05283a3b0146e57b1b639c66ecf2 https://doi.org/10.1134/s0003683812040072 Zobrazit plný text záznamu Plný text ve formátu PDF