Effects of Heyan Kuntai Capsule (和颜坤泰胶囊) on Follicular Development and Oocyte Cohesin Levels in Aged Mice
| Autor: | Hans-Jürgen Gober, Ling Wang, Xuemin Qiu, Da-Jin Li, Nan Chu, Bin Zhang, Wei Tang |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
030219 obstetrics & reproductive medicine TUNEL assay Cohesin Chemistry medicine.medical_treatment H&E stain General Medicine Oocyte Staining Andrology 03 medical and health sciences 030104 developmental biology 0302 clinical medicine medicine.anatomical_structure Complementary and alternative medicine Apoptosis Follicular phase medicine Pharmacology (medical) Saline |
| Zdroj: | Chinese Journal of Integrative Medicine. 24:768-776 |
| ISSN: | 1993-0402 1672-0415 |
| DOI: | 10.1007/s11655-018-2835-3 |
| Popis: | To evaluate the effect of Heyan Kuntai Capsule (和颜坤泰胶囊, HYKT) on the ovarian function of aged mice and expressions of cohesion complexes in oocytes. Twenty-five 9-month-old female C57BL/6J mice were randomly divided into 5 groups by block randomization method (n=5 per group), including the control group (saline), 17β-estradiol group [E2, 100 μg/(kg•d)], and low-, medium-, and highdose of HYKT groups [0.3, 0.9, 2.7 g/(kg•d), respectively]. All mice were treated by intragastric administration for 4 weeks. Hematoxylin and eosin staining and anti-VASA staining were used to detect the amounts of follicles. The apoptosis of follicles was measured by anti-gamma H2A histone family member X (γH2AX) staining and TdT-mediated dUTP Nick-End Labeling (TUNEL) assay. The density of cohesin subunits, REC8 meiotic recombination protein (REC8), structural maintenance of chromosome (SMC) 1β and SMC3 in oocytes were evaluated by immunofluorescent staining. After administration of E2 and high-dose of HYKT, the total number of follicles as well as the number of primordial and primary follicles were significantly increased (P |
| Databáze: | OpenAIRE |
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