Quantification of trovafloxacin in serum by high-performance liquid chromatography with on-line solid-phase extraction

Autor:	S. Bompadre, L. Leone, Sandro Ripa, L. Ferrante
Rok vydání:	1999
Předmět:	Detection limit Chromatography Chemistry Organic Chemistry Clinical Biochemistry Extraction (chemistry) Analytical chemistry Biochemistry High-performance liquid chromatography Analytical Chemistry Blood serum Linear range Sample preparation Solid phase extraction Antibacterial agent
Zdroj:	Chromatographia. 49:185-187
ISSN:	1612-1112 0009-5893
DOI:	10.1007/bf02575283
Popis:	A rapid, simple, accurate and sensitive liquid chromatographic assay with on-line solid-phase extraction is described for determination of trovafloxacin in human serum. Samples were deproteinized with acetonitrile and injected on to an NH2 extraction column for sample clean-up. Thereafter, an on-line column-switching system was used for quantitative transfer of the drug to a C18 analytical column. Separation was performed by ion-pair chromatography and detection was by ultraviolet absorbance at 275 nm. Recovery was 98.5%. The linear range was from 0.25 to 20μg mL−1, with a correlation coefficient of 0.999. Detection limit was 0.1 μg mL−1 from extraction of 25 μL serum.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::107eae4f787cdf00675ff7ebb962d606 https://doi.org/10.1007/bf02575283 Zobrazit plný text záznamu Full text from SpringerLink