Controlling Hyperhydricity in Date Palm In Vitro Culture by Reduced Concentration of Nitrate Nutrients
| Autor: | Maiada M. El-Dawayati, Zeinab E. Zayed |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
0106 biological sciences
0301 basic medicine Hyperhydricity food and beverages 01 natural sciences body regions 03 medical and health sciences Tissue culture chemistry.chemical_compound 030104 developmental biology Nutrient Micropropagation Nitrate chemistry Callus Botany Environmental science Palm 010606 plant biology & botany Explant culture |
| Zdroj: | Methods in Molecular Biology ISBN: 9781493971558 |
| DOI: | 10.1007/978-1-4939-7156-5_15 |
| Popis: | Hyperhydricity (or vitrification) is a fundamental physiological disorder in date palm micropropagation. Several factors have been ascribed as being responsible for hyperhydricity, which are related to the explant, medium, culture vessel, and environment. The optimization of inorganic nutrients in the culture medium improves in vitro growth and morphogenesis, in addition to controlling hyperhydricity. This chapter describes a protocol for controlling hyperhydricity during the embryogenic callus stage by optimizing the ratio of nitrogen salts of the Murashige and Skoog (MS) nutrient culture medium. The best results of differentiation from cured hyperhydric callus are obtained using modification at a ratio of NH4+/NO3- at 10:15 (825:1425 mg/L) of the MS culture medium to remedy hyperhydric date palm callus and achieve the recovery of normal embryogenic callus and subsequent regeneration of plantlets. Based on the results of this study, nutrient medium composition has an important role in avoiding hyperhydricity problems during date palm micropropagation. |
| Databáze: | OpenAIRE |
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