149 EFFECT OF RESVERATROL ANALOGUE ON DEVELOPMENT OF IN VITRO-FERTILIZED BOVINE EMBRYOS
| Autor: | A. D. Silva, J. R. Ribeiro, Carlos Antônio de Carvalho Fernandes, L. S. Amorim, Luiz Sérgio de Almeida Camargo, T. A. Patrocínio, J. H. M. Viana, G. C. Macedo |
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| Rok vydání: | 2017 |
| Předmět: |
02 engineering and technology
Reproductive technology 010501 environmental sciences Resveratrol Biology 01 natural sciences Cryopreservation Andrology chemistry.chemical_compound Endocrinology Human fertilization Genetics medicine Blastocyst Molecular Biology Incubation Fertilisation 0105 earth and related environmental sciences Embryo culture 021001 nanoscience & nanotechnology medicine.anatomical_structure Reproductive Medicine chemistry Immunology Animal Science and Zoology 0210 nano-technology Developmental Biology Biotechnology |
| Zdroj: | Reproduction, Fertility and Development. 29:183 |
| ISSN: | 1031-3613 |
| DOI: | 10.1071/rdv29n1ab149 |
| Popis: | Oxidative stress is one of the main effects of in vitro culture. Generation of reactive oxygen species (ROS) by embryos can be enhanced by the sub-optimal in vitro culture conditions and are associated with a delay in embryonic development. However, supplementation of culture medium with antioxidant agents can minimize the effects of ROS (Guérin et al. 2001 Hum. Reprod. Update 7, 175–189). Resveratrol is an example of a potent antioxidant, and modifications in its structure can improve its biological activity. This study evaluated the effect of AR33 (formula with patent pending), an analogue of resveratrol with high antioxidant activity, on embryo development. Bovine cumulus-oocyte complexes recovered from ovaries collected at the slaughterhouse were in vitro matured for 24 h and oocytes were in vitro fertilized for 20 h, both at 38.8°C under 5% CO2 in air and high humidity. Partially denuded presumptive zygotes were randomly distributed in 4 treatments (with 6 replicates): 0 µM (control, n = 347), 0.1 µM (n = 337), 0.5 µM (n = 277), and 2.5 µM (n = 343) of AR33. The base medium was SOFaa supplemented with 2.5% FCS and incubation conditions were 38.8°C under 5% CO2 in air and high humidity. Half of culture medium was renewed (feeding) at Day 3 and 5 post-fertilization. Cleavage was evaluated at Day 3 and blastocyst rates at Day 7 and 8 post-fertilization. Data were analysed by logistic regression considering the significance level of P 0.05) among 0.1, 0.5, and 2.5 µM (18.1 ± 5.4%, 17.5 ± 2.9%, and 19.4 ± 3.3%, respectively) and all of them were higher (P 0.05) among 0.1, 0.5, and 2.5 µM AR33 (21.0 ± 5.0%, 18.4 ± 2.1%, and 24.6 ± 3.3%, respectively) but only 0.1 and 2.5 µM showed higher (P |
| Databáze: | OpenAIRE |
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