Inflammatory cytokines and related genes are induced in the rat hippocampus by limbic status epilepticus

Autor:	Ada De Luigi, Silvio Garattini, Mirko Conti, Teresa Ravizza, Carlo Perego, Annamaria Vezzani, Maria Grazia De Simoni, D. Moneta, Francesco Marchesi
Rok vydání:	2000
Předmět:	medicine.medical_specialty Necrosis medicine.drug_class General Neuroscience Hippocampus Stimulation Status epilepticus Biology Receptor antagonist Proinflammatory cytokine Nitric oxide synthase Endocrinology Interleukin 1 receptor antagonist nervous system Internal medicine medicine biology.protein medicine.symptom
Zdroj:	European Journal of Neuroscience. 12:2623-2633
ISSN:	0953-816X
DOI:	10.1046/j.1460-9568.2000.00140.x
Popis:	Limbic status epilepticus was induced in rats by unilateral 60-min electrical stimulation of the CA3 region of the ventral hippocampus. As assessed by RT-PCR followed by Southern blot analysis, transcripts of interleukin-1β, interleukin-6, interleukin-1 receptor antagonist and inducible nitric oxide synthase were significantly increased 2 h after status epilepticus in the stimulated hippocampus. Induction was maximal at 6 h for interleukin-1β (445%), interleukin-6 (405%) and tumour necrosis factor-α (264%) and at 24 h for interleukin-1 receptor antagonist (494%) and inducible nitric oxide synthase (432%). In rats with spontaneous seizures (60 days after status epilepticus), interleukin-1β mRNA was still higher than controls (241%). Immunocytochemical staining of interleukin-1β, interleukin-6 and tumour necrosis factor-α was enhanced in glia with a time-course similar to that of the respective transcripts. Sixty days after status epilepticus, interleukin-1β immunoreactivity was increased exclusively in neurons in one third of the animals. Multiple intracerebroventricular injections of interleukin-1 receptor antagonist (0.5 μg/3 μL) significantly decreased the severity of behavioural convulsions during electrical stimulation and selectively reduced tumour necrosis factor-α content in the hippocampus measured 18 h after status epilepticus. Thus, the induction of spontaneously recurring seizures in rats involves the activation of inflammatory cytokines and related pro- and anti-inflammatory genes in the hippocampus. These changes may play an active role in hyperexcitability of the epileptic tissue.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::10763640bdc78ea0f724dcb6b6c47ea6 https://doi.org/10.1046/j.1460-9568.2000.00140.x Zobrazit plný text záznamu Plný text ve formátu PDF Plný text ve formátu HTML
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