| Autor: |
Tanveer Ahmed Shaik, James McMasters, Jürgen Popp, Florian Korinth, Christoph Krafft, Laura Marcu, Anne K. Haudenschild, Martin Richter, Alba Alfonso García |
| Rok vydání: |
2020 |
| Předmět: |
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| Zdroj: |
Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XVIII. |
| DOI: |
10.1117/12.2544225 |
| Popis: |
Genipin cross-linked engineered tissues are 10000 times less toxic than glutaraldehyde cross-linked tissues. Hence, genipin is a better fixative to support the recellularization of tissue-engineered constructs such as vascular grafts. Here, we demonstrate the ability of fiber-based Fluorescence Lifetime Imaging (FLIm) guided Raman spectroscopy to monitor the quality of genipin cross-linked vascular grafts with high speed and specificity. Current results indicate that the fluorescence lifetime of AR-BP shortens upon GE cross-linking. Raman spectroscopy reveals secondary structural changes occurring in the extracellular matrix of pericardia that correspond to Amide I, Amide III and C-C stretch vibrations. We conclude that FLIm guided Raman imaging can detect cross-linking signatures with biochemical specificity and that this imaging modality provides a non-destructive and label-free method to assess the quality of vascular grafts |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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