Advancements in 13C isotope tracking of synergistic substrate co-utilization in Pseudomonas species and implications for biotechnology applications

Autor:	Caroll M. Mendonca, Rebecca A. Wilkes, Ludmilla Aristilde
Rok vydání:	2020
Předmět:	0106 biological sciences 0303 health sciences Chemistry Bioconversion Biomedical Engineering Bioengineering Substrate (biology) 01 natural sciences 03 medical and health sciences Pseudomonas species 010608 biotechnology Biochemical engineering 030304 developmental biology Biotechnology
Zdroj:	Current Opinion in Biotechnology. 64:124-133
ISSN:	0958-1669
DOI:	10.1016/j.copbio.2020.02.004
Popis:	Pseudomonads are well-known to thrive in diverse and complex nutritional habitats, and these capabilities make Pseudomonas species attractive as whole-cell biocatalysts. Industrial bioconversion processes often rely on complex uptake and synergistic metabolic systems due to the presence of varied carbon substrates in nutrient feedstocks. Isotope labeling experiments (ILEs) are emerging techniques used to elucidate cell metabolism following feeding on isotopically enriched substrates and are pivotal to the understanding of carbon partitioning during co-utilization. In this review, we highlight the applications of ILEs to decipher the metabolic networks in Pseudomonas species and evaluate their relevance in optimizing biocatalytic platforms.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::1065ea5ce1e80d09aca2516d491c699c https://doi.org/10.1016/j.copbio.2020.02.004 Zobrazit plný text záznamu Full Text from ScienceDirect