| Autor: | Luit Slooten, Frank Van Breusegem, Dirk Inzé, Guy Bauw, Marc Van Montagu, Johan Botterman, Sergei Kushnir |
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| Rok vydání: | 1998 |
| Předmět: |
Genetically modified maize
biology animal diseases Nicotiana tabacum fungi RuBisCO food and beverages Plant Science General Medicine Chimeric gene biology.organism_classification Chloroplast Gene product Biochemistry Genetics biology.protein Cauliflower mosaic virus Nicotiana plumbaginifolia Agronomy and Crop Science |
| Zdroj: | Plant Molecular Biology. 38:491-496 |
| ISSN: | 0167-4412 |
| DOI: | 10.1023/a:1006032110409 |
| Popis: | Transgenic maize (Zea mays L.) and tobacco (Nicotiana tabacum Petit Havana SR1) plants have been generated, which overproduce a mitochondrial Nicotiana plumbaginifolia manganese superoxide dismutase (MnSOD) in chloroplasts. For this, the mature MnSOD-coding sequence was fused to a chloroplast transit peptide from a Pisum sativum ribulose-1,5-bisphosphate carboxylase (Rubisco) gene and expression of the chimeric gene was driven by the cauliflower mosaic virus (CaMV) 35S promoter. The transgenic MnSOD gene product was correctly targeted to the chloroplasts both in maize and tobacco. However, despite the use of the CaMV 35S promoter, the MnSOD was predominantly localized in the chloroplasts of the bundle sheath cells of maize. Furthermore, the transit peptide was cleaved off at a different position in maize and tobacco. |
| Databáze: | OpenAIRE |
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