| Autor: |
Giovanni R. Vianna, Bárbara B. A. Dias, Margareth M. C. Albino, Francisco J. L. Aragão, Nicolau B. da Cunha, L. J. Cançado, Lı́lian de Mesquita Silva, Josias C. Faria |
| Rok vydání: |
2006 |
| Předmět: |
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| Zdroj: |
Plant Science. 171:565-571 |
| ISSN: |
0168-9452 |
| DOI: |
10.1016/j.plantsci.2006.06.010 |
| Popis: |
The rep gene of Bean golden mosaic virus (BGMV) is essential for virus replication. A mutated rep gene with amino acid codon change in the putative nucleoside triphosphate (NTP) binding motif D262R was created. Phaseolus vulgaris transformation was achieved with a vector that contained the mutated rep and bar genes. A total of 17 initial (T0) transformants were analyzed. One line (M1/4) showed tolerance to glufosinate ammonium and partial resistance to the virus, that is, disease incidence depended on inoculation level. The incidence of BGMV increased with the increasing number of viruliferous whiteflies per plant, both in the transgenic and in the control plants. However, the number of symptomless plants was significantly higher in the transgenic group. The line M1/4 was studied during several generations and presented stability in the transgene loci and virus resistance. Southern blot analysis with genomic DNA of eight generations led to an estimate of two copies of the rep gene integrated at the same locus. RT-PCR analysis revealed the presence of both bar and rep genes transcripts. The mutated REP protein was present in amounts detectable by Western blot analysis in transgenic plants. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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Full Text from ScienceDirect