Studies on Functionalized Chitosan Beads. I. Selective Adsorption of Bovine Lactoferrin onto Partially Sulfated Chitosan Beads
| Autor: | Kaoru Sato, Shin-Ichiro Nishimura, Yoshihide Kawamura, Shun-ichi Dosako, Hiroshi Seo, Seiichi Tokura, Norio Nishi, Koki Itoyama |
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| Rok vydání: | 1993 |
| Předmět: |
chemistry.chemical_classification
Chromatography biology Chemistry Lactoferrin technology industry and agriculture Salt (chemistry) Chemical modification macromolecular substances General Medicine Chitosan chemistry.chemical_compound Adsorption Sulfation Affinity chromatography Selective adsorption biology.protein |
| Zdroj: | Sen'i Gakkaishi. 49:580-585 |
| ISSN: | 1884-2259 0037-9875 |
| Popis: | Porous chitosan beads preparing under the wet condition showed specific capacity to adsorb lactoferrin directly from bovine milk by the partial sulfation reactions. Crosslinking of chitosan beads followed by sulfation was found to give much improved stability and the material exhibited excellent property as an adsorbent material for the affinity chromatography of lactoferrin even in case for higher degrees of sulfation. The amount of lactoferrin bound to the column of chitosan beads was effectively enhanced by increasing CSV value (salt splitting capacity per bed volume of beads) untill 100μeq/mL. |
| Databáze: | OpenAIRE |
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