Evaluation of Plasmodium vivax ELISA for the blood screen
| Autor: | Seong Soo A. An, Chae Seung Lim, Eun-Taek Han, Hee Kyung Lee, Myung Hyun Nam, Kap No Lee, Jang Su Kim, Won Ja Lee, Chi Hyun Cho |
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| Rok vydání: | 2010 |
| Předmět: |
medicine.medical_specialty
Blood transfusion biology medicine.medical_treatment Plasmodium vivax Public Health Environmental and Occupational Health medicine.disease biology.organism_classification Virology Giemsa stain law.invention Apicomplexa Infectious Diseases law parasitic diseases Tropical medicine Immunology biology.protein medicine Parasitology Antibody Malaria Polymerase chain reaction |
| Zdroj: | Tropical Medicine & International Health. 15:1436-1441 |
| ISSN: | 1360-2276 |
| DOI: | 10.1111/j.1365-3156.2010.02657.x |
| Popis: | Summary Plasmodium vivax malaria is the indigenous strain in the Republic of Korea (ROK). Plasmodium vivax can be transmitted through the transfusions of various blood components, which became a severe problem with the safety of blood transfusions and blood-related products in ROK. We evaluated a P. vivax-specific enzyme-linked immunosorbent assay (Genedia Malaria Ab ELISA 2.0, Green Cross, ROK) with blood samples from four groups: 251 samples from P. vivax-infected patients, 39 samples from post-treatment patients upon follow-up, 200 samples from healthy volunteers and 421 samples from domestic travellers to and from high endemic areas of ROK. The positive cases from the ELISA test were confirmed by both Giemsa microscopic and polymerase chain reaction methods. The clinical sensitivity and specificity of detecting P. vivax with ELISA test were 94.4% and 99.0%, respectively. Thirteen of 421 domestic travellers (3.0%) to endemic areas tested positive. The results indicate the effectiveness of detecting antibodies against P. vivax in blood with Genedia Malaria Ab ELISA 2.0 test in a large blood screen setting. |
| Databáze: | OpenAIRE |
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