Conjugative Transfer and Expression of Genes Coding for Periplasmic Nitrate Reductase in the Purple Bacterium Rhodospirillum rubrum

Autor:	Angela Hougardy, Annette Kaminski, Jobst-Heinrich Klemme
Rok vydání:	1996
Předmět:	Rhodobacter Rhodospirillum biology Chemistry Rhodospirillum rubrum Structural gene Periplasmic space biology.organism_classification Nitrate reductase General Biochemistry Genetics and Molecular Biology Microbiology Rhodobacter sphaeroides Plasmid Biochemistry bacteria
Zdroj:	Zeitschrift für Naturforschung C. 51:900-902
ISSN:	1865-7125 0939-5075
DOI:	10.1515/znc-1996-11-1221
Popis:	Plasmid pFR400, a derivative of the vector plasmid pPHU231 containing the structural genes of the periplasmic nitrate reductase (nap genes) of Rhodobacter sphaeroides DSM 158 (F. Reyes et al., Molec. Microbiol. 19, 1307-1318 [1996]) was transferred by conjugative mating to a streptomycin-resistant strain of the nitrate reductase-negative nonsulfur purple bacterium Rhodospirillum rubrum S1. Transconjugant cells of the latter bacterium, identified by their resistance to streptomycin and tetracycline and by their plasmid pattern, contained an active nitrate reductase which, like other periplasmic bacterial nitrate reductases, was not repressed by ammonium.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::10472171363782fc3e0c3463c954d3dc https://doi.org/10.1515/znc-1996-11-1221 Zobrazit plný text záznamu