Immobilization and catalytic properties of candida lipolytic lipase on surface of organic intercalated and modified MgAl-LDHs
| Autor: | Yan Chen, Qinghong Xu, Peiyong Qin, Chunling Ge, Lijun Dong |
|---|---|
| Rok vydání: | 2014 |
| Předmět: |
chemistry.chemical_classification
biology Immobilized enzyme Layered double hydroxides General Chemistry engineering.material Condensed Matter Physics Catalysis chemistry.chemical_compound Enzyme chemistry Triethoxysilane engineering biology.protein Organic chemistry General Materials Science Sodium dodecyl sulfate Lipase Nuclear chemistry Thermostability |
| Zdroj: | Solid State Sciences. 31:8-15 |
| ISSN: | 1293-2558 |
| DOI: | 10.1016/j.solidstatesciences.2014.02.006 |
| Popis: | In this study, MgAl-LDHs (layered double hydroxides) intercalated with sodium dodecyl sulfate and outside surface modified with (3-aminopropyl)triethoxysilane (KH550) were prepared. The existence of organic part in LDHs improved immobilization efficiency and activity recovery of candida lipolytic lipase loaded. Also the positive charge in framework of LDHs was found to be beneficial to the enzyme immobilization. An immobilization efficiency of 56.4% and an activity recovery over 69.2% of the enzyme were obtained after it was loaded on the intercalated and modified LDHs, and catalytic activity of the immobilization can be kept at least five times. Moreover, the immobilized enzyme was found to have higher temperature resistance, wider pH value and better thermostability in reactive activity. |
| Databáze: | OpenAIRE |
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