In vitro Supplementation of Linoleic Acid Improves Quality of Cryopreserved Buffalo Semen

Autor:	I Maqsood, S Shamas, Rabea Ejaz, A U Husna, Asima Azam, S Akhter, B A Rakha, S Qadeer, M S Ansari
Rok vydání:	2020
Předmět:	General Veterinary urogenital system Linoleic acid Artificial insemination medicine.medical_treatment Extender Semen Biology Sperm Cryopreservation law.invention Andrology chemistry.chemical_compound chemistry law medicine Animal Science and Zoology Acrosome Sperm motility
Zdroj:	Indian Journal of Animal Research.
ISSN:	0976-0555 0367-6722
DOI:	10.18805/ijar.b-1182
Popis:	Present study was designed to evaluate the effect of linoleic acid (LA) supplementation in extender on post thaw quality of cryopreserved buffalo semen. Semen was collected from three adult Nili Ravi buffalo bulls of same age with artificial vagina (42°C) for five weeks (replicates; N=30). Qualified semen ejaculates (>1mL volume, >60% motility, >0.5 billion/mL concentration) were diluted in tris-citric acid extender containing 0.0 (control), 5.0, 10.0 and 20.0ng mL-1 of LA and were cryopreserved using standard procedures. Sperm motility and plasma membrane integrity were improved plessthan0.05) in extender containing 10.0 ng mL-1 of LA compared to other treatments and control while number of acrosome intact live sperm, chromatin integrity and number of morphologically normal sperms remained the same. In conclusion, LA supplementation in extender at 10.0 ng mL-1 was found to be beneficial to improve post thaw quality of cryopreserved buffalo semen.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::102cd3427a770d89c40ed872dc9a115b https://doi.org/10.18805/ijar.b-1182 Zobrazit plný text záznamu