Multi-chain action of exo-D-galacturonanase from carrot

Autor:	Jana Perečková, Kveta Heinrichová
Rok vydání:	1983
Předmět:	chemistry.chemical_classification Reaction mechanism Enzyme biology chemistry Chain (algebraic topology) Biochemistry Stereochemistry Exo-D-galacturonanase General Chemistry biology.organism_classification Daucus carota
Zdroj:	Collection of Czechoslovak Chemical Communications. 48:3579-3588
ISSN:	1212-6950 0010-0765
DOI:	10.1135/cccc19833579
Popis:	Two possible modes of action of exo-D-galacturonanase from carrot (E.C. 3.2.1.67) were investigated; this enzyme catalyses the sequential hydrolytic cleavage of pectants and oligogalacturonans by a terminal action from the nonreducing end of the molecule. The experiments indicate that the investigated exo-D-galacturonanase degrades these substrates by a predominantly multi-chain mechanism. Distribution of degradation products of oligomeric substrates (hexa- and pentagalacturonide) under an optimal condition for the action of the enzyme (pH and temperature) indicates that a multi-chain enzyme attack with a prevalent simple collision is involved. Results of the enzyme degradation kinetics are in a good agreement with the above-mentioned presumption.
Databáze:	OpenAIRE
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