Cloning of Sucrose:Sucrose 1-Fructosyltransferase from Onion and Synthesis of Structurally Defined Fructan Molecules from Sucrose1

Autor:	Peter Weisbeek, Andres Wiemken, Edward Smeets, Sjef Smeekens, Antoine Bos, Marcel Lüscher, Anja J.H. Van Dijken, Irma Vijn
Rok vydání:	1998
Předmět:	Sucrose biology Physiology cDNA library fungi food and beverages Plant Science Protoplast biology.organism_classification Tulipa gesneriana chemistry.chemical_compound Fructan Invertase chemistry Biochemistry Complementary DNA Genetics Allium
Zdroj:	Plant Physiology. 117:1507-1513
ISSN:	1532-2548 0032-0889
DOI:	10.1104/pp.117.4.1507
Popis:	Sucrose (Suc):Suc 1-fructosyltransferase (1-SST) is the key enzyme in plant fructan biosynthesis, since it catalyzes de novo fructan synthesis from Suc. We have cloned 1-SST from onion (Allium cepa) by screening a cDNA library using acid invertase from tulip (Tulipa gesneriana) as a probe. Expression assays in tobacco (Nicotiana plumbaginifolia) protoplasts showed the formation of 1-kestose from Suc. In addition, an onion acid invertase clone was isolated from the same cDNA library. Protein extracts of tobacco protoplasts transformed with this clone showed extensive Suc-hydrolyzing activity. Conditions that induced fructan accumulation in onion leaves also induced 1-SST mRNA accumulation, whereas the acid invertase mRNA level decreased. Structurally different fructan molecules could be produced from Suc by a combined incubation of protein extract of protoplasts transformed with 1-SST and protein extract of protoplasts transformed with either the onion fructan:fructan 6G-fructosyltransferase or the barley Suc:fructan 6-fructosyltransferase.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::1017be345a8c7848f0dcfff669dee01e https://doi.org/10.1104/pp.117.4.1507 Zobrazit plný text záznamu