Effective Use of Enzyme Linked Immunosorbent Assay (ELISA) and Polymerase Chain Reaction (PCR) for Detection of Herpes Simplex Virus Infection in Pregnant Females at Sohag University Hospital
| Autor: | Mamdouh M. Esmat, Salah A. Ismail, Hameda Hassan Mohamed |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
chemistry.chemical_classification
Pregnancy Fetus 030219 obstetrics & reproductive medicine biology business.industry University hospital medicine.disease medicine.disease_cause Virology law.invention 03 medical and health sciences 0302 clinical medicine Enzyme Herpes simplex virus chemistry law Immunology medicine biology.protein Seroprevalence 030212 general & internal medicine Antibody business Polymerase chain reaction |
| Zdroj: | The Egyptian Journal of Medical Microbiology. 25:1-7 |
| ISSN: | 1110-2179 |
| DOI: | 10.12816/0037086 |
| Popis: | Background: Infection with herpes simplex virus is one of the most common sexually transmitted infections. Because the infection is common in women of reproductive age it can be contracted and transmitted to the fetus during pregnancy and the newborn, which can lead to death or long-term disabilities. Objectives: The aim of our study was to detect the seroprevalence of Herpes Simplex Virus infection in pregnant females at Sohag University Hospital using Enzyme Linked Immunosorbent Assay (ELISA) confirmed by Polymerase Chain Reaction (PCR) as well as the fetal outcome of infected women in comparison with healthy. Methodology: The study was carried out on 60 pregnant women attending the Obestetric and Gynacology department of Sohag University Hospital fulfilled the inclusion criteria. They were screened for type specific HSV antibodies (HSV-1 IgG and IgM, HSV-2 IgG and IgM) using Enzyme Linked Immunosorbent Assay (ELISA). For IgM positive cases, HSV DNA was detected by Polymerase Chain Reaction (PCR). Blood samples were collected from all the participants after written informed consent. Univariate and multivariate analysis were performed to identify the risk factors associated with HSV positivity. Results: ELISA test for HSV-1 antibodies revealed 14 (23.33%) cases positive for IgM and 44 (73.33%) cases positive for IgG antibodies. For HSV-2 antibodies there were 4 (6.67%) cases positive for IgM and 38 (63.33%) cases positive for IgG antibodies. By PCR HSV-DNA was detected in 14 (77.77%) of the 18 ELISA positive cases representing (23.33%) of the studied populations. Conclusion: According to our study overall prevalence of HSV infection among pregnant women in Sohag University Hospital is about 23%, mostly due to HSV-1. |
| Databáze: | OpenAIRE |
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