A solid-phase enzymeimmunoassay for the determination of progesterone in bovine, porcine and ovine plasma

Autor:	W. D. Sutherland, M. L. Connor, R. P. Del Vecchio
Rok vydání:	1995
Předmět:	Chromatography Plasma samples biology Assay sensitivity Acetylcholine esterase Standard curve chemistry.chemical_compound Food Animals chemistry Blood plasma Monoclonal biology.protein Animal Science and Zoology Petroleum ether Bovine serum albumin
Zdroj:	Canadian Journal of Animal Science. 75:525-529
ISSN:	1918-1825 0008-3984
Popis:	The purpose of this project was to develop a valid quantitative enzymeimmunoassay (EIA) for progesterone in blood plasma of cattle, pigs and sheep. Rabbit anti-progesterone, mouse monoclonal anti-rabbit IgG, authentic progesterone, and acetylcholine esterase bound covalently to progesterone were the principal reagents used to develop the EIA. Ninety-six well microliter plates were coated with mouse monoclonal anti-rabbit IgG and saturated with bovine serum albumin before use. Rabbit anti-progesterone was diluted to a working dilution of 1:2.0 × 106. Standard curves were linear and ranged from 1.56 to 400 pg of progesterone per well which allowed for the measurement of 0.03125 to 8.0 ng mL−1. Assay sensitivity averaged 1.56 pg well−1. Progesterone was extracted from plasma samples with petroleum ether. Plasma samples (n = 3 or 4 from each species) with unknown amounts of progesterone that were extracted and serially diluted with EIA buffer did not deviate from parallelism with progesterone standard curves in buffer. The correlation between EIA and radioimmunoassay (RIA) measurements of progesterone in the same plasma samples was high (P
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::1002e31cc46cd580eef06305dc700e13 https://doi.org/10.4141/cjas95-079 Zobrazit plný text záznamu