SPECTRAL MODIFICATIONS OF BACTERIAL ANTENNA COMPLEXES BY LIMITED PROTEOLYSIS
| Autor: | Herbert Zuber, René A. Brunisholz |
|---|---|
| Rok vydání: | 1993 |
| Předmět: |
Circular dichroism
medicine.diagnostic_test Strain (chemistry) biology Chemistry Proteolysis Analytical chemistry General Medicine biology.organism_classification Biochemistry Crystallography medicine Carboxypeptidase A biology.protein Hypsochromic shift Physical and Theoretical Chemistry Rhodospirillales Antenna (radio) Rhodospirillaceae |
| Zdroj: | Photochemistry and Photobiology. 57:6-12 |
| ISSN: | 1751-1097 0031-8655 |
| Popis: | The spectral effects of a number of endo- and exoproteases to the detergent-solubilized antenna complexes B802-858 (sphaeroides like) of strain Rhodopseudomonas acidophila 10050 and B802-824 of strain 7050 were analyzed. Upon protease digestion the time course of spectral modification between 250 and 900 nm was recorded. In addition, circular dichroism (CD) signals were measured between 450 and 950 nm. The endoprotease elastase as well as the exoprotease carboxypeptidase A (CP A) altered the B802-858 antenna complex, by changing the 858 nm band hypochromically (30-50%) with an additional small hypsochromic shift (3-6 nm) |
| Databáze: | OpenAIRE |
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