| Popis: |
Background: In various herbal formulations, Artocarpus altilis is used to manage diabetes and is listed as an insulin sensitiser. As a result, the current research attempted to identify the likely lead hits to promote glucose uptake using a computational approach, followed by an experimental assessment of a hydroalcoholic extract of the Artocarpus altilis Plant in yeast cells. Methods: The baker yeast was used for the in vitro assay for glucose uptake, while the in silico study involved retrieving phytoconstituents from public sources, predicting for likely targets of diabetes, and then predicting for likely side effects and ADMET profile. In addition, PyRx 0.8's Autodock Vina predicted each ligand's affinity for binding to the glucose transporter. 13 phytoconstituents from Artocarpus altilis were found to have antidiabetic properties. At 500 g/mL, the extract demonstrated the highest glucose uptake in yeast cells. Network pharmacology identified 2, 4, 5', 7'-tetrahydroxyflavanone and 2, 4, 5', 7'-tetrahydroxyflavane phytoconstituents from Artocarpus altilis as specific targets that were modulated in the MAPK signalling pathway, PI3-Akt signalling pathway, and insulin signalling pathway. These targets included INSR, AKT1, PTPN, and IGFR1. Conclusion: Here, the in vitro and in silico methods used in the present study illustrated the potential role of phytoconstituents from Artocarpus altilis in promoting glucose uptake. |
