Molecular confirmation of an absidiomycosis following treatment with a probiotic supplement in a child with leukemia

Autor: B. Bellete, P. Flori, C. Berger, H. Raberin, C. Guy, A. Clemenson, R. Tran Manh Sung, J. Hafid
Rok vydání: 2006
Předmět:
Zdroj: Journal de Mycologie Médicale. 16:72-76
ISSN: 1156-5233
DOI: 10.1016/j.mycmed.2006.03.001
Popis: We report a case of gastro-intestinal absidiomycosis in a 10-year-old girl with leukemia following the use of a probiotic supplement. The patient had a relapse of an acute leukemia treated 6 years ago. She developed an acute appendicitis followed by multiple sub-hepatic abscesses while recovering from a pre-allograft aplasia following chemotherapy. The pathology results for the appendix and the liver abscess showed filamentous fungus compatible with mucoral. Culture was not done for the appendix, and that of the liver abscess was negative. The patient was reported to have ingested Colotium probiotic supplement (Societe ADVITEC, Cholet, France) three times daily in the three preceding weeks to restore her normal intestinal micro-flora. A high concentration of Absidia corymbifera strains and some Aspergillus flavus and Candida spp. were isolated from the contents of the capsule. Using A. corymbifera- specific primers for the 28S rRNA gene and a second pan-fungal primer-pair to amplify and sequence the ITSI and ITSII regions of the rRNA, it was confirmed that the strain from the capsule and that from the liver abscess were A. corymbifera with sequences more than 98% similar. In the absence of a known variable region for epidemiological typing described to-date for this group of organisms, the above evidence highly suggested that the probiotic capsules were the source of infection in the child. Physicians prescribing these supplements, which are manufactured from fermented barley and contain significant quantities of moulds and yeasts, should therefore be aware of the risks involved in immunosuppressed individuals.
Databáze: OpenAIRE
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