Extracellular Matrix from Whole Porcine Heart Decellularization for Cardiac Tissue Engineering
| Autor: | Christopher C. Knutson, Nima Momtahan, Alonzo D. Cook, Matthew Hodgson |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Decellularization Materials science Cellular differentiation 02 engineering and technology CELL DEBRIS 021001 nanoscience & nanotechnology Cell biology Extracellular matrix 03 medical and health sciences 030104 developmental biology Tissue engineering Antigen Porcine heart 0210 nano-technology Induced pluripotent stem cell Biomedical engineering |
| Zdroj: | Methods in Molecular Biology ISBN: 9781493976553 |
| DOI: | 10.1007/7651_2017_31 |
| Popis: | Decellularization of whole porcine hearts followed by recellularization with fully differentiated cells made from patient-specific human induced pluripotent stem cells (iPSCs) may provide the ultimate solution for patients with heart failure. Decellularization is the process of completely disrupting all cells and removing the cellular components (e.g., antigenic proteins, lipids, DNA) from organic tissue, leaving only the extracellular matrix (ECM). The decellularization of porcine hearts can be accomplished in 24 h and results in 98% DNA removal with only 6 h of detergent exposure. Automatically controlling the pressure during decellularization reduces the detergent exposure time while still completely removing immunogenic cell debris. |
| Databáze: | OpenAIRE |
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