Different epigenome regulation and transcriptome expression of CD4 + and CD8 + T cells from monozygotic twins discordant for psoriasis

Autor: Xiaofang Li, Kaiming Zhang, Xinhua Li, Junqin Li, Ruixia Hou, Jianxiao Xing, Xincheng Zhao, Fusheng He, Qiang Wang, Guohua Yin
Rok vydání: 2020
Předmět:
Zdroj: Australasian Journal of Dermatology. 61
ISSN: 1440-0960
0004-8380
Popis: Objectives Psoriasis is an immunodeficient skin disorder, and its exact pathogenesis is unclear. Monozygotic twins are presumed to be genetically identical, and their phenotypic differences may be due to transcriptional regulation or epigenome factors. To explain the inconsistency between twins, we have collected 3 pairs of monozygotic twins who are discordant for psoriasis. Methods Reduced representation of bisulfite sequencing and RNA sequencing was conducted using the peripheral blood of the twins to find the genes playing important roles in psoriasis pathogenesis. Results As a result, we found methylation diversity in four genes (MAST3, MTOR, PM20D1 and ZNF99), and we also found 9 differentially expressed genes (PPAN-P2RY11, PIGV, RPS18, TMEM121, KIF21A, KCNH2, WNT10B, PRX and CDH24) by RNA sequencing. According to the conjoint analysis of methylation and the mRNA results, PTPN6, CCL5, NFATC1 and PRF1 were found to be closely related to psoriasis. We then annotated the genes to explore the associations between these genes and psoriasis. Conclusions These findings provide a better understanding of psoriasis that can improve the diagnosis and treatment of the disease.
Databáze: OpenAIRE
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