Chemical composition, antioxidant activity, neuroprotective and anti-inflammatory effects of cipó-pucá (Cissus sicyoides L.) extracts obtained from supercritical extraction
| Autor: | Priscila do Nascimento Bezerra, Glides Rafael Olivo Urbina, Walace Gomes-Leal, Marcilene Paiva da Silva, R.N. Carvalho Junior, Wandson Braamcamp de Souza Pinheiro, Alessandra Santos Lopes, Marielba de los Angeles Rodriguez Salazar, Vânia Maria Borges Cunha, Jonabeto Vasconcelos Costa |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
Antioxidant
biology Traditional medicine Chemistry medicine.drug_class General Chemical Engineering medicine.medical_treatment 010401 analytical chemistry Extraction (chemistry) Supercritical fluid extraction Condensed Matter Physics biology.organism_classification 01 natural sciences Neuroprotection Anti-inflammatory 0104 chemical sciences Terpene 03 medical and health sciences 0302 clinical medicine medicine Cissus Physical and Theoretical Chemistry Chemical composition 030217 neurology & neurosurgery |
| Zdroj: | The Journal of Supercritical Fluids. 138:36-45 |
| ISSN: | 0896-8446 |
| Popis: | Cissus sicyoides L. is a plant used in the treatment of stroke and abscesses in popular Brazilian medicine. The aim of this work was to determine the best operational condition of the supercritical fluid extraction (SFE) of C. sicyoides with CO2 and CO2 + EtOH using the Box–Behnken design based on global yield, phenolic compounds, and flavonoids and antioxidant activity, compared to conventional extraction (Soxhlet), and to evaluate the extract’s neuroprotective effect. The optimal process condition corresponded to 400 bar, 40 °C, and 10% EtOH. Pressure and the cosolvent percentage were the factors that influenced SFE. Extracts with increased antioxidant activity were obtained in both extractions (SFE and Soxhlet). HPTLC analysis of the extracts showed the presence of terpenes, phenolic compounds, and flavonoids. The preliminary in vivo assay showed that the extract obtained by SFE has a neuroprotective and anti-inflammatory effect in a model of focal cerebral ischemia. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect