Virulence potential of two entomopathogenic nematodes, their associated bacteria, and its metabolites to larvae of Pieris brassicae L. (Lepidoptera, Pieridae) in cabbage under greenhouse and field bioassays

Autor: Waseem Abbas, Imran Ul Haq, Nazir Javed, Sohail Ahmed
Rok vydání: 2021
Předmět:
Zdroj: International Journal of Tropical Insect Science. 42:557-563
ISSN: 1742-7592
Popis: The cabbage butterfly (Pieris brassicae L.) is a major insect pest of cabbage in Pakistan. Heterorhabditis bacteriophora (at 1500 infective juvenile/ ml concentraction), Steinerma glaseri (at 1500 infective juvenile/ ml concentration), H. bacteriophora + S. glaseri (at 1500 infective juvenile/ ml concentration), Xenorhabdus spp., (at 4 × 1012 concentration) Photorhabdus spp., (at 4 × 1012 concentration) metabolites of Xenorhabdus spp. (at 50% concentration) and metabolites of Photorhabdus spp (at 50% concentration) were sprayed on cabbage hybrid CB-60 grown in pots placed in the greenhouse. After 7 days, the highest P. brassicae larval mortality (90.43%) was observed where H. bacteriophora + S. glaseri were sprayed. In field experiment, best-performing treatments from greenhouse experiment were selected i.e. H. bacteriophora + S. glaseri (at 1500 infective juvenile/ ml concentration), Xenorhabdus spp., (at 4 × 1012 concentration) and metabolites of Xenorhabdus spp. (at 50% concentration) were sprayed on a highly susceptible cabbage hybrid (CB-60). Heterorhabditis bacteriophora + Steinerma. glaseri @ 1500 infective juvenile/ ml was found to be more superior with maximum mortality (80.43) of cabbage butterfly larvae in comparison with other treatments. From the present study, it may be concluded that EPNs (H. bacteriophora and S. glaseri) application is more effective as compared to their symbiotic bacteria and metabolite for cabbage butterfly management.
Databáze: OpenAIRE