| Popis: |
This study investigated whether GLP-1 affects islet β cells in high glucose and the possible mechanism. INS-1 cells are separated into group A (treated with 5.6 mmol/L glucose), group B (group A+100 nmo1/L liraglutide), group C (cells were cultured in 16.7 mmol/L glucose) and group D (group C+100 nmo1/L liraglutide) followed by analysis of cell activity by CCK-8, apoptosis by Hoechst staining, INS level by ELISA, AMPK/mTOR signaling protein level by immunoblotting and RT-PCR. Compared with group A, cell proliferation was significantly increased in group B (P P P P P P P |
