The myxoid liposarcoma specificTLS-CHOPfusion protein localizes to nuclear structures distinct from PML nuclear bodies
| Autor: | Alondra Schweizer Burguete, Melker Göransson, Pierre Åman, Anita Olofsson, Sofia Thelin-Järnum |
|---|---|
| Rok vydání: | 2001 |
| Předmět: |
Transcription Factor CHOP
Cancer Research Myxoid liposarcoma genetic structures biology CHOP medicine.disease eye diseases Cell biology Cell nucleus Promyelocytic leukemia protein medicine.anatomical_structure Oncology immune system diseases hemic and lymphatic diseases polycyclic compounds medicine Cancer research biology.protein Nuclear protein Nuclear localization sequence RNA-Binding Protein FUS |
| Zdroj: | International Journal of Cancer. 97:446-450 |
| ISSN: | 0020-7136 |
| DOI: | 10.1002/ijc.1632 |
| Popis: | CHOP in 12q13, also called GADD153 or DDIT3, encodes a transcription factor of the C/EBP type. As a result of t(12;16) translocations, CHOP is rearranged and fused to TLS in 16p11 in about 90% of myxoid liposarcomas/round cell liposarcomas (MLS/RCLS). The TLS-CHOP protein consists of the N-terminal half of TLS juxtaposed to the N-terminal of the entire CHOP. It is capable of forming dimers with the natural dimer partners of CHOP. Here we report that recombinant TLS-CHOP-green fluorescence protein localizes to nuclear structures, similar to, but distinct from, PML nuclear bodies. The TLS-CHOP-green fluorescent protein nuclear structures are resistant to high salt concentration and nuclease treatment. Transfection of TLS-CHOP to normal fibroblasts causes a rapid down regulation and relocation of PML nuclear bodies. An abnormal extra nuclear localization of PML bodies was also found in TLS-CHOP carrying cell lines established from myxoid liposarcomas. Transfection of TLS-CHOP induced a rapid disappearance of PCNA. TLS-CHOP may disturb the nuclear machinery by binding and sequestering important factors from their natural sites. |
| Databáze: | OpenAIRE |
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